Dynamic Oxygen Enhances Oocyte Maturation in Long-Term Follicle Culture
نویسندگان
چکیده
منابع مشابه
Secondary follicle growth and oocyte maturation during encapsulated three-dimensional culture in rhesus monkeys: effects of gonadotrophins, oxygen and fetuin.
BACKGROUND An alginate-based matrix supports the three-dimensional (3D) architecture of non-human primate follicles and, in the presence of FSH, permits the in vitro development of pre-antral follicles to the small antral stage, including the production of ovarian steroids and paracrine factors. The current study investigated the ability of gonadotrophins, fetuin and oxygen (O₂) to improve prim...
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This paper reviews two recent developments in the field of assisted reproduction: in-vitro culture (IVC) of follicles and in-vitro maturation (IVM) of oocytes. Both in-vitro procedures for oocytes and immature follicles were developed so as to enable storage of ovarian cortical tissue and oocyte-cumulus complexes (OCC) from small follicles. Until now, complete in-vitro development from a primor...
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In vitro follicle growth is a potential approach to preserve fertility for young women who are facing a risk of premature ovarian failure (POF) caused by radiation or chemotherapy. Our two-step follicle culture strategy recapitulated the dynamic human follicle growth environment in vitro. Follicles developed from the preantral to antral stage, and, for the first time, produced meiotically compe...
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An option for fertility preservation for women facing a cancer diagnosis involves the cryopreservation of ovarian tissue for later re-transplantation or in vitro culture, with in vitro culture preferred to avoid reintroduction of the cancer. Small, immature follicles survive the freeze-thaw process, and can be matured through in follicle maturation (IFM) that involves an initial growth of the f...
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ژورنال
عنوان ژورنال: Tissue Engineering Part C: Methods
سال: 2009
ISSN: 1937-3384,1937-3392
DOI: 10.1089/ten.tec.2007.0418